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目的 设计一种带有通用序列标签的寡核苷酸探针以应用于寡核苷酸芯片点样的质量控制.方法 以HIV寡核苷酸探针作为核心序列,在其一端或两端连接上多种通用序列标签(UST)构建新型探针.然后打印芯片,以Cy5标记的通用引物(Cy5-UP,与UST互补)进行杂交,筛选出5条荧光信号强的探针,重新打印芯片.再分别以不同浓度梯度的、Cy5标记的通用引(Cy5-UP)和随机引(Cy5-RP)与芯片杂交,比较两者杂交效率.结果 Cy5-Up的杂交结果显著优于Cy5-RP的杂交结果,特别是在较低浓度时.结论 利用Cy5标记的通用引物检测探针上的通用序列标签,可以为芯片点样的质量控制提供一种更有效的方法.“,”Aim To design an universal sequence-tagged oligonucleotide probes for spot quality control of oligonucleotide mieroarrays.Methods HIV oligo probes used as core target sequences were linked with several universal sequence tags (USTs) at their one or both ends,and were then printed onto the shdes.Cy5-labeled universal primers(Cy5-UP)which were complementary to the USTs were hybridized to the arrays.Five probes with stable strong signals were selected,printed again and hybridized with Cy5-UP and Cy5-labeled random primers(Cy5-RP)at different concentration levels,respectively.Results The signals of Cy5-UP were remarkably stronger than those of Cy5-RP,especially at a low concentration level.Conclusion The results demonstrated that the detection of the USTs on the probes by hybridization using Cy5-UP will provide a more effective method for spot QC of microarrays.