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作者研究根尖周炎和牙髓炎的病原菌,检查各型根尖周炎加重期20例,慢性牙髓炎19例,病人年龄18～63岁。利用捲在根管探针或拔髓针上的无菌棉拴取根管内容物,在治疗椅旁立即放入培养厌氧菌的半液状培养基内,同时在2个富有血琼脂的培养皿内培养,1个放在恒温器内37℃培养需氧菌,另1个放在厌氧菌培养器,抽出空气并在器内充满氢、氮和二氧化碳混合气体;为结合剩余氧,利用钯催化剂或硷-焦性没食子酚混合物。根据培养物的形态学特点和生化性质,鉴定分离出的厌氧菌,用常法确定兼性厌氧菌种的属性。研究结果根尖周炎20例,根管内容物在厌氧条件下有细菌生长18例,需氧条件下有细菌生长只6例。共分离出37个菌株,其中非梭形芽胞杆菌属厌氧菌29株,兼性厌氧菌8株。厌氧菌:消化链球菌11株,胨球菌6株,梭菌6株,厌氧杆菌5株,韦永氏球菌2株。
The author studied pathogens of periapical periodontitis and pulpitis, examined 20 cases of periapical period exacerbations, 19 cases of chronic pulpitis, and patients aged 18 to 63 years. The contents of the root canal were tied with aseptic cotton wound on a root canal probe or a puller, immediately placed in a semi-liquid culture medium for culturing anaerobes near the treatment chair, and cultured in two agar-agar-rich One dish was incubated in a thermostat at 37 ° C for aerobic bacteria and the other on an anaerobic incubator. The air was extracted and filled with a mixture of hydrogen, nitrogen and carbon dioxide. In order to combine the residual oxygen, Palladium catalyst or a base-pyrogallol mixture. According to the morphological characteristics and biochemical properties of the culture, the isolated anaerobic bacteria were identified, and the properties of facultative anaerobe species were determined by the usual method. The results of 20 cases of apical periodontitis, root canal contents under anaerobic bacteria growth in 18 cases, aerobic bacteria growth in only 6 cases. A total of 37 isolates were isolated, of which 29 were anaerobic bacteria and 8 were facultative anaerobes. Anaerobic bacteria: Peptostreptococcus 11, Peptococcus 6, Clostridium 6, 5 strains of Anaerobic bacteria, 2 of Weiyou bacteria.