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慢乙肝患者肝细胞核内嗜伊红包涵体、经Biauchi氏应用电镜和免疫荧光检查,认为是由于HBcAg颗粒过多堆积形成。本文研究慢乙肝细胞核内嗜伊红物质(LNS)与血清HBV-DNA之间的关系。试图探讨较简易检测肝细胞核内HBcAg方法。25例肝组织每一标本经石蜡包埋后连续切片,分别用HE、苯胺兰、PAS糖元染色、Feulgen、Orcein等方法染色,最后光镜检查评价。同时检测甲、乙肝血清学标志,对照组:血清抗-HAVIgM~+急性坏死型肝炎1例,其余3例为正常肝组织。结果:20例慢性乙型肝炎之肝组织经苯胺蓝染色后,肝细胞核质着色呈不同程度之3组阳性核(N~+):
Eosinophils in hepatocytes of patients with chronic hepatitis B were electron microscopy and immunofluorescence examination by Biauchi’s method, which was considered to be due to excessive accumulation of HBcAg particles. This article studies the relationship between the eosinophilic substance (LNS) in the nucleus of chronic hepatitis B virus and serum HBV-DNA. Trying to explore a simpler method of detecting HBcAg in liver cells. Twenty-five specimens of liver tissue were embedded in paraffin and cut into sections. The specimens were stained with HE, Aniline, PAS glycogen, Feulgen and Orcein, respectively. Finally, they were evaluated by light microscopy. Serological markers of A and B were detected at the same time. In the control group, 1 case of anti-HAVIgM + acute necrotic hepatitis and 3 cases of normal liver tissue were detected in the control group. Results: Twenty liver tissues of chronic hepatitis B were stained with aniline blue and the nuclei of hepatocytes were stained with three groups of positive nuclei (N ~ +) to varying degrees: