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目的研究排钱草生物碱对乙醛刺激的人源肝星状细胞(LX-2)的增殖抑制作用及其对细胞外基质生成的影响。方法采用MTT法检测排钱草生物碱对乙醛刺激下人源肝星状细胞的增殖的作用;LDH比色法检测排钱草生物碱对LX-2的细胞毒性;运用Real-time PCR技术检测细胞中α-SMA、Co1-Ⅰ、MMP-2及TIMP-1 mRNA的表达情况。结果排钱草生物碱对乙醛刺激下的肝星状细胞的增殖具有明显抑制作用,呈剂量依赖性。排钱草生物碱各剂量组培养上清液中LDH活力差异无显著性。LX-2细胞中的α-SMA、Co1-Ⅰ、及TIMP-1 mRNA随药物浓度增加,其表达下降,MMP-2 mRNA表达上升。结论排钱草生物碱可能通过抑制LX-2细胞的增殖和减少细胞外基质的合成,从而发挥抗肝纤维化作用。
Objective To study the inhibitory effects of Desmodium pulchricorn alkaloids on the proliferation of human hepatic stellate cells (LX-2) stimulated by acetaldehyde and its effect on extracellular matrix production. Methods MTT assay was used to determine the effect of the alkaloids from Desmodium pulchellus on the proliferation of human hepatic stellate cells stimulated by acetaldehyde. The LDH colorimetric method was used to detect the cytotoxicity of the desmodium pulchellum alkaloids on LX-2. Real-time PCR The expression of α-SMA, Co1-Ⅰ, MMP-2 and TIMP-1 mRNA in the cells were detected. Results The destroyer alkaloids significantly inhibited the proliferation of hepatic stellate cells stimulated by acetaldehyde in a dose-dependent manner. There was no significant difference in the activity of LDH in the supernatant of each row of cashmere alkaloids. The expression of α-SMA, Co1-Ⅰ and TIMP-1 mRNA in LX-2 cells decreased with the increase of the drug concentration, and the expression of MMP-2 mRNA increased. Conclusion Rowan alkaloids may exert anti-hepatic fibrosis effects by inhibiting the proliferation of LX-2 cells and reducing the synthesis of extracellular matrix.