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以盆栽小白菜、萝卜和辣椒为试材,对其根际土壤采用磷脂脂肪酸(PLFA)含量分析、土壤理化性质分析方法,分为清水与清水+镉(Cd)2种处理,研究了不同处理对蔬菜根际土壤微生物群落的影响。结果表明:1)清水+Cd处理的蔬菜根际土壤微生物的含量均无显著差异,但萝卜根际土壤的微生物含量与小白菜和辣椒相比较低。2)主成分分析表明,用清水+Cd灌溉处理蔬菜,对萝卜根际土壤微生物群落结构影响最大。3)不同灌溉方式处理下的蔬菜根际土壤微生物类群中PLFA的含量均表现为革兰氏阳性菌(G+)>革兰氏阴性菌(G-)>放线菌>真菌;与清水对照相比,用含Cd的溶液灌溉蔬菜,其PLFA值均无显著差异。4)用不同灌溉方式浇灌蔬菜,在蔬菜收获期对土壤理化性质进行分析表明,清水+Cd处理对小白菜吸收土壤中的全氮及萝卜和辣椒吸收土壤中的硝酸盐氮的影响较大。
Using potted cabbage, radish and pepper as test materials, the content of phospholipid fatty acid (PLFA) in soil rhizosphere soil and the analysis method of soil physical and chemical properties were divided into two groups: fresh water and fresh water + cadmium (Cd) Effects of Rhizosphere Soil Microbial Communities on Vegetables. The results showed that: 1) There was no significant difference in the rhizosphere soil microbial contents between the fresh water and the Cd-treated vegetables, but the rhizosphere soil microbial biomass was lower than the cabbage and pepper. 2) Principal component analysis showed that the treatment of vegetables with water + Cd had the greatest effect on the rhizosphere soil microbial community structure. The contents of PLFA in the rhizosphere soil microbial communities under different irrigation methods showed Gram-positive bacteria (G +)> Gram-negative bacteria (G-)> actinomycetes> fungi; Compared with the solution containing Cd irrigation vegetables, PLFA values were no significant difference. 4) The irrigation of vegetables by different irrigation methods, the analysis of soil physical and chemical properties during the harvest period of vegetables showed that the effect of fresh water + Cd on total nitrogen absorbed by Chinese cabbage and nitrate nitrogen in radish and pepper absorbed soil.