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目的了解并分析深圳市龙岗区腹泻患者粪便、禽肉类等样品中空肠弯曲菌的流行状况及同源性。方法对2016年5—12月深圳市龙岗区某医院腹泻患者粪便或肛拭子样品和来自养鸡场、农贸市场、超市等地的禽畜类等样品进行空肠弯曲菌分离培养,菌株经镜检和生化鉴定后再使用脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)对其进行全基因组分子分型。最后应用Bio Numerics软件进行聚类分析。结果 41份腹泻患者样品分离出5株空肠弯曲菌,分离率为12.2%,全部阳性样品均来自11岁以下儿童。53份来自食品和外环境样品分离出14株空肠弯曲菌,分离率为26.4%,其中来自农贸市场的分离率为57.1%(8/14),来自不同超市样品空肠弯曲菌的分离率为11.1%(4/36)。18株菌呈现15种PFGE带型,其中来自市场1的3株菌株带型完全一致,且与病例1只有一条带的差异;来自市场2与病例5的带型完全一致且与病例4的带型只有一条带的差异。结论深圳市龙岗区腹泻患者空肠弯曲菌感染率较高,感染可能是由于食用了来自市场未煮熟的食物或接触了来自市场被空肠弯曲菌污染的食物,农贸市场存在空肠弯曲菌的交叉污染,该地区空肠弯曲菌呈现多样性。
Objective To understand and analyze the prevalence and homology of Campylobacter jejuni in the samples of diarrhea patients and diarrhea in Longgang District of Shenzhen City. Methods Samples of feces or anal swabs from diarrhea patients in a hospital in Longgang District of Shenzhen City from May to December in 2016 and Campylobacter species in farms, farmer’s markets and supermarkets were isolated and cultured. The whole genome was genotyped by pulsed field gel electrophoresis (PFGE) after biopsy and biochemical identification. Finally, Bio Numerics software was used for cluster analysis. Results Five strains of Campylobacter jejuni were isolated from 41 samples of diarrhea patients, with a separation rate of 12.2%. All the positive samples were from children under 11 years of age. Fifty-three strains of Campylobacter jejuni were isolated from 53 samples of food and environmental samples, with a separation rate of 26.4%. The isolation rate from the farmer’s market was 57.1% (8/14). The separation rate of Campylobacter jejuni from different supermarkets was 11.1 % (4/36). The 18 isolates showed 15 PFGE patterns. Among them, the three isolates from market 1 were identical in band pattern and had only one band difference with case 1; the patterns from market 2 and case 5 were identical with those of case 4 Only one type of band difference. Conclusion The prevalence of Campylobacter jejuni is high in diarrhea patients in Longgang District of Shenzhen City. The infection may be caused by eating uncooked food from the market or with food contaminated with Campylobacter jejuni from the market. Cross-contamination of Campylobacter jejuni exists in the farmer’s market , Campylobacter jejuni diversity in the region.