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目的 :用 99Tcm 标记的人体内蛋白 annexin V进行凋亡细胞的体外检测和体内显像。方法 :1Jurkat T原始淋巴细胞采用无血清培养基培养0~ 4天 ( n=10 )、阿霉素 0~ 2 0 0 ng/ml孵育 48小时 ( n=10 )和 CHll Fas抗体 0~ 2 5 ng/ml孵育 2 4小时 ( n=8)等方法处理 (诱导细胞凋亡
OBJECTIVE: To detect in vitro and in vivo imaging of apoptotic cells with human annexin V (99Tcm). Methods: The primary lymphocytes of Jurkat T were cultured in serum-free medium for 0-4 days (n = 10), adriamycin (0-20 000 ng / ml) for 48 hours ng / ml incubation 24 hours (n = 8) and other methods (induction of apoptosis