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目的建立测定人工养殖蟒蛇胆汁中牛磺蟒胆酸钠含量的方法。方法采用高效液相色谱法测定其含量。色谱条件:Phenomenex Luna C18色谱柱(250 mm×4.60 mm,5μm);流动相为乙腈-0.15%磷酸氢二钠(10%磷酸调pH至3.0),梯度洗脱;流速为1.0 mL.min-1;检测波长203 nm;柱温35℃;进样量10μL。结果牛磺蟒胆酸钠在20.2~322.4μg.mL-1(r=0.998 9)浓度范围内线性关系良好,平均回收率为102.4%(RSD=1.4%,n=6);8批蟒蛇胆汁中牛磺蟒胆酸钠含量差异较大,平均含量为65.09 mg/g-1。结论本方法准确、快速、灵敏、重复性好,可用于蟒蛇胆汁的质量控制。
Objective To establish a method for determination of sodium taurocholate sodium in cultured biliary pythons. Methods The content was determined by high performance liquid chromatography. Chromatographic conditions: Phenomenex Luna C18 column (250 mm × 4.60 mm, 5 μm); mobile phase of acetonitrile-0.15% disodium hydrogen phosphate (10% phosphoric acid adjusted to pH 3.0), gradient elution; flow rate of 1.0 mL.min- 1; detection wavelength 203 nm; column temperature 35 ℃; injection volume 10μL. Results A good linear relationship was found in the range of 20.2-332.4 μg.mL-1 (r = 0.998 9) with an average recovery of 102.4% (RSD = 1.4%, n = 6). Eight batches of python bile The contents of sodium taurocholate in sodium differed greatly with an average content of 65.09 mg / g-1. Conclusion The method is accurate, rapid, sensitive and reproducible. It can be used for the quality control of python bile.