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目的：观察体外培养的大鼠肾小球系膜细胞在内毒素（ＬＰＳ）诱导下ＩＬ┐６的产生及其ｍＲＮＡ表达。方法：按经典方法培养肾小球系膜细胞（ＭＣ）、转种培养后分为二组，诱导组加ＬＰＳ１０ｍｇ／Ｌ，对照组不加，用分子生物学和免疫学方法进行检测。结果：ＩＬ┐６在正常ＭＣ中分泌量极微，其ｍＲＮＡ也为低水平表达，经ＬＰＳ诱导后二者均明显增加。ＩＬ┐６蛋白的产生６～１２ｈ达高峰，比对照组增加３～５倍，ＩＬ┐６ｍＲＮＡ的表达３～６ｈ达高峰，较对照组增加２～３倍。结论：ＬＰＳ可诱发系膜细胞ＩＬ┐６水平增高，可能作为炎症介质参与肾脏疾病的发病，在系膜增殖性肾炎发病中起一定作用
OBJECTIVE: To observe the IL-6 production and its mRNA expression induced by endotoxin (LPS) in cultured rat glomerular mesangial cells. Methods: The mesangial cells (MC) were cultured according to the classical method and divided into two groups after induction of culture. The induced group was treated with LPS 10 mg / L, while the control group was not added. The molecular biology and immunological methods were used to detect the mesangial cells. Results: IL-6 secreted in normal MC was extremely low, and its mRNA was also low-level expression, both of which were significantly increased after induced by LPS. The production of IL-6 protein peaked at 6 to 12 hours and increased 3 to 5 times than that in control group. The expression of IL-6 mRNA peaked at 3 to 6 hours, which was 2 to 3 times higher than that in control group. CONCLUSION: LPS can induce an elevated level of IL-6 in mesangial cells, which may play an important role in the pathogenesis of mesangial proliferative glomerulonephritis as an inflammatory mediator