论文部分内容阅读
首次采用以改性纤维素为基质、亚氨二乙酸（ＩＤＡ）为取代配基的铜离子螯合膜色谱法对牛肝过氧化氢酶（ＢＬＣ）的分离纯化进行了研究。缓冲液的ｐＨ值对ＢＬＣ与螯合配基的结合影响显著。在选定的色谱条件下，ＢＬＣ粗酶液经ＩＤＡ型Ｃｕ２＋－螯合膜色谱柱一步纯化，比活性平均提高４．７倍，回收率为６７．７％。金属螯合膜色谱柱可用含０．２ｍｏｌ／Ｌ的咪唑或５０ｍｍｏｌ／ＬＥＤＴＡ－１ｍｏｌ／ＬＮａＣｌ的缓冲液再生，反复使用，后者比前者对柱子的再生效果更好。
The isolation and purification of bovine liver catalase (BLC) was first studied by Cu2 + chelate membrane chromatography with modified cellulose as matrix and iminodiacetic acid (IDA) as the substituted ligand. The pH of the buffer has a significant effect on the binding of BLC to the chelating ligand. Under the selected chromatographic conditions, the BLC crude enzyme solution was purified in one step by Cu2 + - chelating membrane with IDA. The specific activity increased by 4.7 times and the recovery rate was 67.7%. The metal chelate membrane column can be regenerated with 0.2mol / L imidazole or 50mmol / L LEDTA-1mol / LNaCl buffer for repeated use. The latter is better than the former for column regeneration.