论文部分内容阅读
目的:观察白藜芦醇联合姜黄素对体外人肝癌细胞SMMC-7721增殖和凋亡的影响及相关信号通路。方法:不同浓度白藜芦醇、姜黄素及两药联合干预SMMC-7721细胞,MTT法检测细胞增殖,流式细胞术检测细胞凋亡、Hoechst 33258染色检测细胞凋亡形态变化,比色法检测半胱氨酸天冬氨酸蛋白酶(caspase)-3,caspase-8,caspase-9酶活性,Western blot法检测半胱氨酸天冬氨酸蛋白酶切割底物(PARP)。结果:与对照组相比,白藜芦醇、姜黄素单独或联合作用SMMC-7721细胞均可抑制SMMC-7721细胞增殖,两药联合后抑制作用更显著。白藜芦醇、姜黄素联合较单独用药可增强SMMC-7721细胞凋亡,呈现凋亡形态改变,白藜芦醇、姜黄素及联合组细胞凋亡率分别为(17.39±1.41)%,(14.96±2.23)%,(25.36±2.68)%;同时提高SMMC-7721细胞caspase-3,caspase-8及caspase-9活性,促使PARP蛋白剪辑。结论:白藜芦醇、姜黄素联合使用可增强对人肝癌细胞SMMC-7721的抗癌作用,并可能与caspase-8,caspase-9/caspase-3/PARP信号通路介导细胞凋亡相关。
Objective: To observe the effects of resveratrol and curcumin on the proliferation and apoptosis of human hepatocellular carcinoma cell line SMMC-7721 in vitro and the related signaling pathways. Methods: SMMC-7721 cells were treated with different concentrations of resveratrol, curcumin and two drugs. Cell proliferation was measured by MTT assay. Apoptosis was detected by flow cytometry. Morphological changes of apoptosis were detected by Hoechst 33258 staining. Caspase-3, caspase-8 and caspase-9 activities were detected by enzyme linked immunosorbent assay (ELISA). The caspase cleavage substrate (PARP) was detected by Western blot. Results: Compared with the control group, resveratrol and curcumin alone or in combination could inhibit the proliferation of SMMC-7721 cells. The combination of resveratrol and curcumin inhibited the proliferation of SMMC-7721 cells more significantly. Resveratrol and curcumin could increase the apoptosis of SMMC-7721 cells compared with the control alone. The apoptotic rates of resveratrol, curcumin and the combination group were (17.39 ± 1.41)% and ( 14.96 ± 2.23)% and (25.36 ± 2.68)%, respectively. At the same time, the activities of caspase-3, caspase-8 and caspase-9 in SMMC-7721 cells were increased and the PARP protein was edited. CONCLUSION: The combination of resveratrol and curcumin can enhance the anticancer effect on SMMC-7721 hepatoma cells and may be related to the apoptosis of caspase-8 and caspase-9 / caspase-3 / PARP signaling pathway.