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目的对云南省1例输入性疟疾患者的血样进行实验室检测分析。方法采用疟原虫属特异性引物和种特异性引物对该样本进行巢式PCR检测,将PCR扩增结果进行序列测定,并对获得的序列进行Blast比对,分析其分子性状。结果使用疟原虫属特异性引物r PLU5和r PLU6对血样进行PCR检测,第一轮未扩增出预期条带;使用疟原虫种特异性引物对血样进行PCR检测,扩增出预期大小约141bp的三日疟条带,无对应大小的恶性疟、间日疟和卵形疟扩增条带产生。序列分析表明,扩增片段长度为115bp,进行Blast比对发现,与Genbank中注册编号为gb|KJ934253.1的三日疟对应部分的基因序列同源性为96%。结论通过使用巢式PCR、序列测定分析等方法,基本证实该病例为三日疟原虫感染。
Objective To analyze and analyze the blood samples of one case of imported malaria in Yunnan Province. Methods The samples were detected by nested PCR using Plasmodium specific primers and species-specific primers. The PCR amplification results were sequenced. The obtained sequences were analyzed by Blast comparison, and their molecular traits were analyzed. Results Plasmodium-specific primers rPLU5 and rPLU6 were used for PCR detection of blood samples. The first round of PCR was not amplified. PCR was carried out on the blood samples using specific primers of Plasmodium species to amplify the expected size of about 141 bp Of the three malaria band, no corresponding size of falciparum malaria, vivax malaria and oval malaria amplification bands. Sequence analysis showed that the amplified fragment was 115bp in length. Blast analysis showed that the sequence was 96% homologous to the corresponding part of P. malaria registered in Genbank (gb | KJ934253.1). Conclusion By using nested PCR and sequence analysis, it was basically confirmed that the case was Plasmodium malariae infection.