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以纤维素粉为唯一碳源,从棉籽壳中筛选出能分解纤维素(CMC)的霉菌,利用常规方法鉴定为绿色木霉,通过紫外线诱变提高分解纤维素的能力,采用水解圈和滤纸崩溃法进行初筛,摇瓶培养复筛的方法,筛选出一突变绿色木霉(T118),降解纤维素的能力提高了1倍,CMC酶活性提高了50%,C1酶活性提高了40%,还对该突变株产酶条件进行了研究,得出最适条件为:稻草粉:麸皮=4:1,发酵时间为5d,发酵温度为30℃,该酶最适反应PH为6,最适反应温度为50℃。此突变株易培养,有利于工业生产,具有重大的实用价值。
Using cellulose powder as the sole carbon source, the fungus that can decompose cellulose (CMC) was screened from the cottonseed shell, identified as Trichoderma viride by conventional methods, and its ability to decompose cellulose was improved by UV mutagenesis. (T118), the ability of degrading cellulose was doubled, the CMCase activity increased by 50% and the C1 enzyme activity increased by 40% The optimum fermentation conditions were as follows: rice straw powder: bran = 4: 1, fermentation time 5d, fermentation temperature 30 ℃, the optimum reaction pH of the enzyme was 6, The optimum reaction temperature is 50 ℃. This mutant is easy to culture, is conducive to industrial production, has great practical value.