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目的:观察血虚证大鼠肠系膜微循环变化,并测量其血液流速。方法:通过皮下注射乙酰苯肼(APH)制备血虚证大鼠模型,运用BI-2000医学图像分析系统观测血虚大鼠肠系膜微循环损伤的变化,采集微循环显微图像,并利用LabVIEWIMAQ VISION平台进行图像处理分析,提供一种跟踪白细胞团的微循环血液流速测量方法。结果:①血虚大鼠肠系膜微循环血色暗淡,微血管变形,管壁受损;②测得的大鼠肠系膜微循环血液流速与现代文献研究十分吻合,精确度提高,再现性良好。③血虚模型组大鼠肠系膜微循环血液流态呈粒线流甚至聚集流改变,且血液流速较正常组明显减慢,具有显著性差异(P<0.05)。结论:血虚大鼠微循环存在供血不足、血流瘀滞、管壁损伤和渗出出血等多重病理改变;运用LabVIEW图像处理方法测量血液流速,客观地反映了血虚大鼠微循环的病理改变,表明显微医学图像分析技术在中医血虚证研究中具有良好的应用前景。
Objective: To observe the changes of mesenteric microcirculation in blood deficiency rats and measure the blood flow velocity. Methods: The rats with blood deficiency syndrome were prepared by subcutaneous injection of acetophenhydrazine (APH). The changes of mesenteric microcirculation injury in blood-deficient rats were observed by BI-2000 medical image analysis system. The microcirculation microscopic images were collected and analyzed with LabVIEWIMAQ VISION Platform for image processing analysis to provide a method of tracking microcirculation blood flow measurement of white blood cells. Results: (1) Mesenteric microcirculation in blood deficiency was bleak, with microvascular degeneration and vascular wall damage. (2) The measured mesenteric microcirculation blood flow velocity was in good agreement with modern literatures. The accuracy and repeatability of the model were good. (3) The blood flow of mesenteric microcirculation in blood deficiency model group changed in granular flow or aggregation flow, and the blood flow velocity was significantly slower than that in normal group (P <0.05). Conclusion: The microcirculation of blood deficiency rats have multiple pathological changes such as insufficient blood supply, blood stasis, wall damage and exudative hemorrhage. Using the LabVIEW image processing method to measure the blood flow velocity, it objectively reflects the pathological changes of microcirculation Change, indicating that microscopic medical image analysis technology has good application prospects in the study of blood-deficiency syndrome of traditional Chinese medicine.