论文部分内容阅读
目的从采自中国黄海的威海海域的柄海鞘来源放线菌Streptomyces coelicoflavus(HQA809)和史氏菊海鞘来源放线菌Nocardiopsis dassonvillei(HQA404)中分离、鉴定具有生物活性的次级代谢产物。方法运用硅胶柱层析、Sephadex LH-20凝胶柱层析和半制备HPLC等方法分离化合物,运用核磁、质谱等现代波谱分析方法对化合物进行结构鉴定,并对化合物进行抗菌、卤虫致死、α-糖苷酶抑制活性和细胞毒活性评价。结果从Streptomyces coelicoflavus(HQA809)中分离鉴定了2个吡喃酮类化合物(1,2),从Nocardiopsis dassonvillei(HQA404)中分离鉴定了1个吩嗪类化合物(3)和1个苯酚类化合物(4);化合物3对鳗弧菌(Vibrio anguillarum)和副溶血弧菌(Vibrio parahaemolyticus)显示出较强的抑菌活性,其MIC值分别为50μmol/L和25μmol/L。结论海鞘来源放线菌可以作为发现新颖的生物活性物质的潜在来源。
OBJECTIVE To isolate and identify bioactive secondary metabolites from Streptomyces coelicoflavus (HQA809) and Nocardiopsis dassonvillei (HQA404) collected from the seaweed of the Yellow Sea in the Weihai Sea of China. Methods The compounds were isolated by silica gel column chromatography, Sephadex LH-20 column chromatography and semi-preparative HPLC. The structures of the compounds were identified by the methods of nuclear magnetic resonance spectroscopy and mass spectrometry. The compounds were antibacterial, Evaluation of α-glucosidase inhibitory activity and cytotoxic activity. Results Two pyranones (1, 2) were isolated and identified from Streptomyces coelicoflavus (HQA809). One phenazine compound (3) and one phenolic compound (1) were isolated and identified from Nocardiopsis dassonvillei (HQA404) 4). Compound 3 showed strong antibacterial activity against Vibrio anguillarum and Vibrio parahaemolyticus with MIC values of 50μmol / L and 25μmol / L, respectively. Conclusion The actinomycetes originating from sea squirt may serve as a potential source for the discovery of novel bioactive substances.