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本研究以普通小麦（ＴｒｉｔｉｃｕｍａｅｓｔｉｖｕｍＬ．；２ｎ＝６ｘ＝４２）品种Ｆｕｋｕｈｏｋｏｍｕｇｉ为母本，以无融合生殖披碱草（Ｅ．ｒｅｃｔｉｓｅｔｕｓ（ＮｅｓｉｎＬｅｈｍ）Ａ．ＬｏｖｅｅｔＣｏｎｎｏｒ；２ｎ＝６ｘ＝４２）品系１０５０为父本进行杂交，利用胚拯救技术，对杂种幼胚进行愈伤组织诱导、植株再生，获得了生长正常的属间杂种Ｆ１。对所获杂种在幼苗期用ＲＡＰＤ标记进行了鉴定，结果表明，有２１个引物的扩增产物Ｆ１呈现共显性，占扩增引物总数的２０．５９％；有６１个引物的扩增产物Ｆ１偏向父本，占扩增引物总数的５９．８０％；有２０个引物的扩增产物Ｆ１偏向母本，占扩增引物总数的１９．６１％。这表明杂种Ｆ１为真杂种。这一属间杂种的获得为进一步开展Ｅ．ｒｅｃｔｉｓｅｔｕｓ无融合生殖基因向小麦转移奠定了基础。本研究结果证明，ＲＡＰＤ标记可作为一种小麦属间杂种快速、有效、简便的分子鉴定方法。
In this study, Fukuhokomugi (Triticum aestivum L.; 2n = 6x = 42) was used as the female parent and 1050 as the male parent of E.rectisetus (NesinLehm) A. Loveet Connor; 2n = 6x = 42) The embryo rescue technique was used to induce the hybrid immature embryos and regenerate the plants, and the normal intergeneric hybrid F1 was obtained. The hybrids were identified by RAPD markers in the seedling stage. The results showed that 21 of the amplified products showed co-dominant, accounting for 20.59% of the total number of amplification primers; amplification products of 61 primers F1 biased toward the male parent, accounting for 59.80% of the total number of amplification primers; the amplification product F1 with 20 primers was biased toward the female parent, accounting for 19.61% of the total number of amplification primers. This shows that hybrid F1 is a true hybrid. This intergeneric hybrid was acquired for further development. rectisetus apomixis gene transfer to wheat laid the foundation. The results of this study demonstrate that the RAPD marker can be used as a rapid, effective and simple molecular identification method for wheat interspecific hybrids.