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目的:探讨经复方苦参注射液处理肝癌细胞(HepG2)后survivin mRNA表达。方法:采用MTT比色法测定肝癌细胞对氧化苦参碱的敏感性。RT-PCR检测survivin mRNA的表达。Western-blot检测survivin蛋白表达的变化。结果:复方苦参注射液处理后的第1天,3组肝癌细胞的survivin mRNA表达水平均降低;其中0.1 g.mL-1复方苦参注射液组下降了5%,0.2 g.mL-1复方苦参注射液组下降8%,0.3 g.mL-1组下降了28%。第3天,0.1 g.mL-1组和0.2 g.mL-1组HepG2细胞的survivin mRNA表达与第1天比较,分别下降10%和36%,0.3 g.mL-1组仍持续降低,为第1天的66%。0.1 g.mL-1组和0.2 g.mL-1组作用3 d后的HepG2细胞中survivin蛋白含量分别下降了3%和15%,0.3 g.mL-1组则下降到了55%。结论:复方苦参通过调控survivin mRNA在肝癌细胞的表达,可能是其抗肿瘤作用的机制。
Objective: To investigate the expression of survivin mRNA in HepG2 cells treated with Compound Kushen Injection. Methods: MTT assay was used to determine the sensitivity of hepatoma cells to oxymatrine. The expression of survivin mRNA was detected by RT-PCR. Western-blot detection of survivin protein expression changes. Results: On the first day after compound Kushen injection, the expression of survivin mRNA in three groups of hepatocellular carcinoma cells was decreased. Among them, 0.1 g.mL-1 compound Kushen injection group decreased by 5%, 0.2 g.mL-1 Compound Kushen injection group decreased 8%, 0.3 g.mL-1 group decreased by 28%. On the third day, the expression of survivin mRNA of HepG2 cells in 0.1 g.mL-1 group and 0.2 g.mL-1 group decreased by 10% and 36% respectively compared with that on the first day, 66% of the first day. The survivin protein content in HepG2 cells treated with 0.1 g · mL-1 and 0.2 g · mL-1 groups decreased by 3% and 15%, respectively, and decreased to 55% in 0.3 g · mL-1 group. Conclusion: Sophora flavescens can regulate the expression of survivin mRNA in hepatoma cells, which may be the mechanism of its anti-tumor effect.