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Three cationic lipids with lysylated(1),histidylated(2),and arginylated(3) headgroups and cholesterol hy-drophobic moiety were synthesized.The average sizes of liposomes and lipoplexes were around 100 and 160 nm,re-spectively.The gene transfection efficiency of the three lipoplexes loaded with pGL3 or pORF-LacZ was compared on 293T cells in the presence or the absence of serum.The transfection efficiency of the three lipoplexes in a se-rum-free medium was 2 to 3-fold higher than that of dioleoyl-trimethylammonium propane(DOTAP).In the presence of serum,however,most of the lipoplexes showed lower transfection activities;only lipoplex 3 retained its high transfection efficiency.
Three cationic lipids with lysylated (1), histidylated (2), and arginylated (3) headgroups and cholesterol hy-drophobic moiety were synthesized. The average sizes of liposomes and lipoplexes were around 100 and 160 nm, re-spectively. The gene transfection efficiency of the three lipoplexes loaded with pGL3 or pORF-LacZ was compared on 293T cells in the presence or the absence of serum. The transfection efficiency of the three lipoplexes in a se-rum-free medium was 2 to 3-fold higher than that of the dioleoyl-trimethylammonium propane (DOTAP) .In the presence of serum, however, most of the lipoplexes showed lower transfection activities; only lipoplex 3 retained its high transfection efficiency.