近年来蛋白质顺序测定在自动化、微量化与策略方面都有显著的进展。用DNA顺序分析技术间接地测定蛋白质顺序的策略已被广泛地采用,它能加快但不能代替蛋白质的顺序测定。质谱技术测定蛋白质顺序的途径也有了可喜的突破。高效液相色谱分离肽技术的发展、限制性酶切及各种选择性的化学与酶的降解方法的出现,为蛋白质顺序测定提供了更多可选用的工具。 In recent years, the determination of protein sequence in the automation, micro-quantification and strategy have made significant progress. Strategies for determining the order of proteins indirectly using DNA sequence analysis techniques have been widely adopted and can speed up, but not replace, the order of proteins. Mass spectrometry has also been a welcome breakthrough in determining the order of proteins. The development of HPLC separation of peptides, restriction enzyme digestion and various selective chemical and enzymatic degradation methods have provided more optional tools for protein sequence determination.