论文部分内容阅读
目的:观察肿瘤转移抑制基因Maspin(mammary-serpin)在宫颈鳞状细胞癌和癌前病变组织中的表达,探讨其在宫颈鳞癌发生、演进中的作用及其意义。方法:运用免疫组织化学Envision二步法检测Maspin在正常宫颈组织、宫颈上皮内瘤变(CIN)和宫颈鳞癌组织的表达情况,进行统计学分析,并比较与宫颈鳞癌临床病理特征的关系。结果:Maspin在正常宫颈组织、CIN和宫颈鳞癌中阳性表达率分别为100.0%、87.5%、50.0%,表达呈逐级下降,宫颈鳞癌与前两者间差异有统计学意义,P<0.01。正常宫颈组织和CIN以细胞质表达为主,宫颈鳞癌以胞核、胞质表达为主,在部分高级别CIN和微小浸润性宫颈癌表达呈异质性,基底区和浸润灶组织阳性信号减弱或缺失。Maspin的表达与宫颈癌的临床分期,肿瘤浸润深度、淋巴结转移及患者的生存时间呈负相关。结论:Maspin表达的下调可能在宫颈鳞癌的发生和演进过程中起一定作用,是观察宫颈CIN进展的一种潜在的有用标志,并与宫颈鳞癌浸润转移和生存密切相关,有望作为判断宫颈癌预后有价值的生物学指标。
Objective: To observe the expression of the tumor metastasis suppressor gene Maspin (mammary-serpin) in cervical squamous cell carcinoma and precancerous lesions, and to explore the role and significance of Maspin (mammary-serpin) in the occurrence and progression of cervical squamous cell carcinoma. Methods: The expression of Maspin in normal cervical tissue, cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma was detected by immunohistochemical Envision two-step method. Statistical analysis was performed and the relationship between Maspin and clinicopathological features was analyzed . Results: The positive expression rates of Maspin in normal cervical tissue, CIN and cervical squamous cell carcinoma were 100.0%, 87.5% and 50.0%, respectively. The expression of Maspin was decreased step by step, the difference between the two groups was statistically significant (P < 0.01. Cervical squamous cell carcinoma was mainly expressed in nucleus and cytoplasm. Normal cervical tissue and CIN were mainly expressed in cytoplasm. Cervical squamous cell carcinoma was mainly expressed in nucleus and cytoplasm. It was heterogeneous in some high-grade CIN and micro-invasive cervical carcinoma. The positive signal in basal and infiltrating foci was weakened Or missing. The expression of Maspin was negatively correlated with clinical stage, depth of tumor invasion, lymph node metastasis and survival time of patients with cervical cancer. Conclusion: The down-regulation of Maspin expression may play a role in the occurrence and progression of cervical squamous cell carcinoma, which is a potential useful marker to observe the progression of cervical CIN. It is closely related to the infiltration, metastasis and survival of cervical squamous cell carcinoma, Prognosis of cancer biological value of valuable indicators.