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用细胞裂解计数法及超薄切片电镜观察法分析了迟缓爱德华氏菌侵袭HEp—2细胞的基本特性。在15株来源各异的迟缓爱德华氏菌中,有6株细菌具有对HEp—2细胞的侵袭能力。细菌侵入细胞后,主要位于空泡内。侵入细胞内的迟缓爱德华氏菌不仅可在细胞内增殖,而且可从细胞内释放出来。用细胞松弛素破坏微丝后可抑制其侵袭作用,而且表现出剂量依赖关系,而用秋水仙素破坏微管后不影响其侵袭力。这表明在迟缓爱德华氏菌对HEp—2细胞的侵袭过程中,细胞骨架中有微丝的参与,未发现微管的参与。
The basic characteristics of HEp-2 cells infected with R. edwardsianus were analyzed by cell lysis counting and ultra-thin section electron microscopy. Among 15 strains of Edwardsiella difficile different from each other, 6 strains had the ability to invade HEp-2 cells. Bacteria invade cells, mainly located in the vacuole. Incertuous Edwardsiella infiltrating cells not only proliferate within the cell, but also release from the cell. Cytochalasin destructive actin filaments can inhibit its invasion, and showed a dose-dependent manner, and colchicine damage colchicine does not affect its invasiveness. This indicates that microtubules are involved in the involvement of microfilaments in the cytoskeleton during the delayed attack of Edwardsiella on HEp-2 cells.