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目的监测近年来广东和江西口岸出入境人员中,艾滋病病毒1型(HIV-1)感染者感染毒株的亚型流行分布特征,为有效的开展口岸艾滋病监测和防控提供科学依据。方法从205份确证的HIV-1抗体阳性样本中提取病毒核酸,通过反转录和巢式PCR技术扩增HIV-1基因的env、p17、vif、vpr和pol区域,将PCR产物测序后进行分析对比,以确定其基因亚型。结果 205份样品中有93份样品扩增阳性,其中广东口岸88份,江西口岸5份。共发现17种HIV-1亚型和重组亚型。其中,A亚型6份(6.5%);B亚型6份(6.5%);C亚型10份(10.8%);D、F、G亚型各1份,分别占1.1%;CRF01_AE重组型33份(35.5%);CRF02_AG重组型12份(12.9%);CRF07_BC重组型13份(14.0%);CRF08_BC重组型3份(3.2%)。此外,研究还发现7个独特重组型(URFs),分别是A/H/J/U、CRF01_AE/A/C、CRF01_AE/B、CRF01_AE/C、CRF02_AG/A、CRF07_BC/A和CRF07_BC/B,这些新的独特重组型是在广东口岸首次发现。这些不同亚型的感染者来自包括中国在内的29个不同国家。结论近年来广东口岸和江西口岸出入境人员中HIV-1亚型和重组亚型种类呈明显的多样化和复杂化。监测出入境人员中HIV感染者基因型分布情况,对及时发现新型毒株,预防和控制艾滋病从国境口岸的传入十分重要。
Objective To monitor the epidemiological distribution of subtypes of HIV-1 infected persons in entry and exit of Guangdong and Jiangxi ports in recent years and provide a scientific basis for the effective monitoring and prevention and control of port AIDS. Methods The viral nucleic acids were extracted from 205 valid HIV-1 antibody positive samples. The env, p17, vif, vpr and pol regions of the HIV-1 gene were amplified by reverse transcription and nested PCR. The PCR products were sequenced Analysis and comparison to determine its genetic subtype. Results Of the 205 samples, 93 samples showed positive amplification, of which 88 were from Guangdong and 5 from Jiangxi. A total of 17 HIV-1 subtypes and recombinant subtypes were found. Among them, 6 subtypes (6.5%) of A subtype, 6 subtypes (6.5%) of B subtype, 10 subtypes of C subtype (10.8%), 1 subtype of D, F and G, accounting for 1.1% (35.5%), 12 (12.9%) CRF02_AG recombinant, 13 (14.0%) CRF07_BC recombinant and 3 (3.2%) CRF08_BC recombinant. In addition, the study also found that seven unique recombinants (URFs) were A / H / J / U, CRF01_AE / A / C, CRF01_AE / B, CRF01_AE / C, CRF02_AG / A, CRF07_BC / A and CRF07_BC / B, These new unique reorganization types were first discovered in the port of Guangdong. Infected by these different subtypes came from 29 different countries, including China. Conclusions In recent years, the types of HIV-1 subtypes and recombinant subtypes in Guangdong ports and Jiangxi ports have obviously diversified and complicated. Monitoring the distribution of genotypes among HIV-infected persons in and out of the country is crucial to discovering new strains in a timely manner and preventing and controlling the spread of AIDS from the border crossings.