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以二棱大麦（Ｈｏｒｄｅｕｍ ｖｕｌｇａｒｅ Ｌ．）“苏啤１ 号”为母本与四倍体球茎大麦（Ｈ． ｂｕｌｂｏｓｕｍＬ．）“ＧＢＣ１４１”杂交， 并通过幼胚培养获得１１ 株三倍体Ｆ１ 植株． 三倍体Ｆ１ 植株与二倍体母本二棱大麦“苏啤１ 号”回交， 获得７ 株二倍体回交后代ＢＣ１． ７ 个回交后代株系通过大麦黄花叶病病圃鉴定， 其中ＢＣ１－２株系抗大麦黄花叶病． 利用聚丙烯酰胺凝胶电泳对父本、母本和ＢＣ１－２株系进行了同工酶分析，结果表明二倍体二棱大麦与四倍体球茎大麦的过氧化物酶同工酶差异显著，并且在ＢＣ１－２株系幼根的过氧化物酶同工酶谱中，发现一条来自父本球茎大麦“ＧＢＣ１４１”的过氧化物酶谱带，该酶带的相对迁移率（Ｒｆ）为０．４７， 重复性好并且易于检测．由于回交后代ＢＣ１－２抗大麦黄花叶病， 又带有来自球茎大麦特异的幼根过氧化物酶谱带作为易于检测的同工酶标记，因此该回交后代株系可以作为大麦抗病育种工作重要的抗源．
Hybrids of Hordeum vulgare L. “Supou 1” with H. bulbosum L. “GBC141” were obtained and eleven triploid F1 plants were obtained through immature embryo culture. Triploid F1 plants were backcrossed with the diploid female barley “Supi 1” to obtain seven diploid backcross BC1. Seven backcrossed progeny lines were identified by barley yellow mosaic disease nursery, in which BC1-2 lines were resistant to barley yellow mosaic disease. Isozyme analysis of the male and female parents and BC1-2 lines by polyacrylamide gel electrophoresis showed that there was significant difference in the peroxidase isozymes between diploid barley and tetraploid bulblets , And in the peroxidase isoenzyme profile of the radicle of BC1-2 strain, a peroxidase band was found from the parental bulb barley “GBC141” with a relative mobility (Rf) of 0.47, repeatable and easy to detect. Backcross progeny lines can be used as barley for disease resistance due to backcrossing offspring BC1-2 against barley yellow mosaic disease and with a band of barley-specific peroxidase from barley as an easy-to-detect isozyme marker Breeding work is an important source of resistance.