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目的探讨慢性活动性Epstein-Barr病毒(Epstein-Barr virus,EBV)感染、急性EBV感染及正常儿童EBV-DNA及适应性体液免疫的差异。方法慢性活动性EBV感染患儿8例(慢性组),急性EBV感染患儿13例(急性组),正常儿童12例(对照组)外周血单个核细胞采用实时荧光定量PCR法检测,3组EBV-DNA水平,采用ELISA、抗体稀释试验评价EBV适应性体液免疫,分析其与EBV感染不同转归的关系。结果慢性组EBV-DNA载量、病毒壳蛋白抗原-IgA、-IgG及早期抗原-IgA水平明显高于急性组和对照组(P<0.01),病毒壳蛋白抗原-IgM水平及早期抗原-IgG滴度改变速率低于急性组(P<0.01);慢性组与急性组EBV核抗原-IgG抗体水平均低于对照组(P<0.01)。结论 慢性活动性EBV感染存在不同EBV核抗原-LP拷贝数及不同亲和力的抗体谱,可能与慢性活动性病程相关,对早期识别诊治有重要意义。
Objective To investigate the differences of Epstein-Barr virus (EBV) infection, acute EBV infection and EBV-DNA and adaptive humoral immunity in normal children. Methods Chronic active EBV infection in 8 children (chronic group), acute EBV infection in 13 children (acute group), 12 normal children (control group) peripheral blood mononuclear cells using real-time fluorescence quantitative PCR method, 3 groups EBV-DNA levels, EBV-adaptive humoral immunity was evaluated by ELISA and antibody dilution test, and its relationship with EBV infection was analyzed. Results The levels of EBV-DNA, antigenic capsular antigen-IgA, -IgG and early antigen-IgA in chronic group were significantly higher than those in acute group and control group (P <0.01). The antigenicity of IgM and IgE The rate of change of titer was lower than that of acute group (P <0.01). The levels of EBV nuclear antigen-IgG in chronic group and acute group were lower than those in control group (P <0.01). CONCLUSION: There are different antibody profiles of different EBV nuclear antigen-LP copies and different affinities in chronic active EBV infection, which may be related to chronic active disease course, which is of great significance for early diagnosis and treatment.