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目的 :观察还原型谷胱甘肽(reduced glutathione hormone,GSH)对重症急性胰腺炎(severe acute pancreatitis,SAP)大鼠海马神经元的保护作用。方法:72只SD大鼠分为3组:生理盐水组(NS组)、SAP组和GSH组,每组又分为3、6、12 h 3个亚组。SAP组以5%牛磺胆酸钠逆行注入大鼠胰胆管建立SAP模型,NS组仅向胰胆管注射与SAP组等量的无菌生理盐水,GSH组在造模后按每100 g体重腹腔内注射25 mg GSH。分别采用尼氏染色检测大鼠脑组织海马区神经元损伤情况,TUNEL法检测海马神经元凋亡情况,免疫组化法检测海马组织NF-κB p65的表达。结果:与SAP组比较,GSH组在GSH治疗后3、6 h,胰腺病理评分明显改善(P均<0.05),海马神经元凋亡指数明显下降(P均<0.05)。海马NF-κB p65表达受抑,尤以GSH组GSH治疗6 h后明显。结论:GSH在SAP脑损伤的早、中期可以通过抑制大鼠海马组织NF-k B p65蛋白的表达来减轻神经元凋亡。
Objective: To observe the protective effect of reduced glutathione hormone (GSH) on hippocampal neurons in rats with severe acute pancreatitis (SAP). Methods: Seventy-two SD rats were divided into three groups: NS group, SAP group and GSH group. Each group was divided into three subgroups: 3,6,12 h. The SAP group was infused with 5% sodium taurocholate retrogradely into the pancreaticobiliary duct of rats to establish the SAP model. NS group was injected with the same amount of sterile saline as the SAP group only in the pancreaticobiliary duct. GSH group was injected intraperitoneally Inject 25 mg GSH. The damage of hippocampal neurons in rat brain was detected by Nissl staining. The apoptosis of hippocampal neurons was detected by TUNEL method. The expression of NF-κB p65 in hippocampus was detected by immunohistochemistry. Results: Compared with SAP group, the pathological scores of pancreatic tissue in GSH group were significantly improved at 3 h and 6 h after GSH treatment (all P <0.05), and apoptosis index of hippocampal neurons decreased significantly (all P <0.05). The expression of NF-κB p65 was inhibited in hippocampus, especially after GSH GSH treatment for 6 h. Conclusion: GSH can attenuate the neuronal apoptosis by inhibiting the expression of NF-κB p65 protein in hippocampus of rats in early and middle SAP.