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目的探讨不同粒径、不同浓度的纳米TiO_2对鼠成纤维细胞的遗传毒性效应。方法分别用含有0μg/ml(对照组)、1μg/ml、10μg/m1、100μg/m1,浓度的30nm和50nm的Ti0_2的DMEM培养基培养鼠成纤维细胞Balb/c3T3,24h后,采用单细胞凝胶电泳实验方法检测DNA损伤,评价毒性效应。结果与对照组比较,30nm和50nm的Ti0_2颗粒当浓度大于1μg/ml时均可对细胞产生遗传毒性效应,差异显著(P<0.05),且随着浓度的增加毒性作用增强,当TiO_2浓度为1 00μg/ml时毒性作用最强。结论纳米二氧化钛对小鼠成纤维细胞具有遗传毒性作用,且遗传毒性与纳米TiO_2浓度呈剂量依赖效应。
Objective To investigate the genotoxic effects of nano-TiO2 with different particle sizes and different concentrations on murine fibroblasts. Methods Balb / c3T3 fibroblasts were cultured in DMEM medium containing 0μg / ml (control group), 1μg / ml, 10μg / ml and 100μg / ml concentrations of 30nm and 50nm Ti0_2 for 24 hours. Gel electrophoresis was used to detect DNA damage and to evaluate the toxic effect. Results Compared with the control group, Ti0 2 particles at 30 nm and 50 nm showed genotoxic effects on cells when the concentration was higher than 1 μg / ml (P <0.05), and the toxicity increased with the increase of the concentration of TiO 2 1 00μg / ml, the strongest toxicity. Conclusion Nano-titania has a genotoxic effect on mouse fibroblasts, and the genotoxicity is in a dose-dependent manner with the concentration of nano-TiO 2.