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由8-甲酰基-7-羟基香豆素与碳酰肼经一步缩合反应可制得探针1。研究发现,探针1对Zn~(2+)和F-离子均呈现荧光增强和比率比色的高灵敏和高选择性响应,检出限低至10-8 mol·L~(-1)。通过光谱、ITC、1H NMR滴定及质谱分析,详细地研究了探针与离子形成的配合物性质。在不同的介质中,探针不仅同时表现出对金属阳离子Zn~(2+)和对阴离子F-的识别,而且吸收和发射波长均有显著的差异:1-Zn~(2+)配合物的最大吸收波长为360 nm,而1-F-配合物为400 nm;1-Zn~(2+)配合物的荧光激发和发射波长分别为360和454 nm,而1-F-配合物分别为400和475 nm。此外,探针1还能应用于活体PC3细胞中Zn~(2+)的荧光成像。
Probe 1 can be prepared by one-step condensation of 8-formyl-7-hydroxycoumarin and carbohydrazide. The results showed that probe 1 showed high sensitivity and selectivity for fluorescence enhancement and ratio colorimetric detection for both Zn 2+ and F- ions with a detection limit as low as 10-8 mol·L -1 . The properties of complexes formed by probes and ions were studied in detail by spectroscopy, ITC, 1H NMR titration and mass spectrometry. In different media, the probe not only showed the recognition of metal cation Zn 2+ and anion F-, but also had significant differences in absorption and emission wavelength: 1-Zn 2+ complex The maximum absorption wavelength was 360 nm, while the 1-F-complex was 400 nm. The fluorescence excitation and emission wavelengths of 1-Zn 2+ complexes were 360 and 454 nm, respectively, while 1-F- For 400 and 475 nm. In addition, probe 1 can also be applied to fluorescence imaging of Zn 2+ in living PC3 cells.