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目的通过在兔腹部复制缺损创面并于创基放置硅胶垫片,用背阔肌肌皮瓣带蒂移植进行修复,构建一种新型的肌皮瓣带蒂移植模型。方法采用新西兰大耳白兔20只,在兔腹部设计软组织缺损创面约6.0 cm×8.0 cm,创基覆盖硅胶垫片,背阔肌肌皮瓣带蒂转移修复腹部创面。按移植术后时间的不同进行分组,每组5只。观察肌皮瓣的大体存活情况、肌纤维的组织病理学改变及肌瓣中微血管密度的改变。结果大体观察各组肌皮瓣均存活良好,无皮瓣坏死,取材时见肌肉外观红润。HE及Masson染色结果显示各组肌纤维形态良好。各组间微血管计数6周>5周>4周>3周,各组内微血管计数近端>中段>远端,差异有统计学意义(P<0.05)。结论以胸背血管为蒂的兔背阔肌肌皮瓣移植修复腹部创面是一种理想的肌皮瓣移植模型,适用于药物、细胞及血运重建等实验研究。
OBJECTIVE: To construct a new model of pedicled myocutaneous pedicel transplantation by means of replicating the defect wound in the abdomen of rabbits and placing a silicone pad on the base of the pedicel and repairing it with pedicled latissimus dorsi myocutaneous flap. Methods Twenty New Zealand white rabbits were used to design a soft tissue defect wound in the abdomen of rabbit about 6.0 cm × 8.0 cm. The foundation was covered with silicone pad and the latissimus dorsi muscle flap pedicled and transferred to repair the abdominal wound. According to the different time after transplantation groups, 5 in each group. Observe the general survival of myocutaneous flap, histopathological changes of muscle fibers and changes in microvessel density in the muscle flap. Results In general, all myocutaneous flaps survived well without skin flap necrosis. HE and Masson staining results showed that each group of muscle fibers in good shape. The microvessel counts in each group were 6 weeks> 5 weeks> 4 weeks> 3 weeks, and the microvessel count in each group was close to the middle> the distal> the middle. The difference was statistically significant (P <0.05). Conclusions The repair of the abdominal wound using the latissimus dorsi latissimus dorsi musculocutaneous flap is an ideal muscle flap transplantation model, which is suitable for the experimental study of drugs, cells and revascularization.