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目的优化小鼠肺炎支原体ELISA检测方法,降低非特异性反应。方法肺炎支原体热灭活处理后制备ELISA板,检测小鼠血清,与常规ELISA及分离培养方法相比较。结果优化后的ELISA方法平均本底值为0.03,敏感度为1:1280;优化ELISA方法与分离培养方法的符合率为99.27%,比常规ELISA方法高;PCR验证改进后的ELISA方法具有较好的准确性。结论优化后的方法准确性好,灵敏度高,对小鼠肺炎支原体的检测具有重要的实际意义。
Objective To optimize the ELISA method for mycoplasma pneumoniae in mice to reduce the nonspecific reaction. Methods Mycoplasma pneumoniae was heat inactivated to prepare ELISA plate, and the serum of mice was detected, compared with the conventional ELISA and isolation culture method. Results The optimized ELISA method had an average background value of 0.03 and a sensitivity of 1: 1280. The coincidence rate of the optimized ELISA method and the isolation and culture method was 99.27%, which was higher than that of the conventional ELISA method. PCR method showed that the improved ELISA method was better Accuracy Conclusion The optimized method has good accuracy and high sensitivity, which has important practical significance for the detection of Mycoplasma pneumoniae in mice.