In the present study,cultured human SHG-44 glioma cells were subjected to a hypoxic environment simulated using the CoCl2 method.Flow cytometry showed increased reactive oxygen species production in these cells.Real-time reverse transcription-PCR showed significantly increased hypoxia-inducible factor-1α mRNA expression in cells exposed to the hypoxic condition.The antioxidant N-acetylcysteine significantly inhibited reactive oxygen species production and reduced hypoxia-inducible factor-1α mRNA expression in normoxic and hypoxic groups,especially in the latter group.These findings indicate that hypoxia induces reactive oxygen species production and hypoxia-inducible factor-1α mRNA expression in human SHG-44 glioma cells,and that the antioxidant N-acetylcysteine can inhibit these changes. In the present study, cultured human SHG-44 glioma cells were subjected to a hypoxic environment simulated using the CoCl2 method. Flow cytometry showed increased reactive oxygen species production in these cells. Real-time reverse transcription-PCR showed significant increased hypoxia-inducible factor -1α mRNA expression in cells exposed to the hypoxic condition. The antioxidant N-acetylcysteine ​​significantly inhibited reactive oxygen species production and reduced hypoxia-inducible factor-1α mRNA expression in normoxic and hypoxic groups, especially in the latter group. These inotropic conditions that hypoxia induces reactive oxygen species production and hypoxia-inducible factor-1α mRNA expression in human SHG-44 glioma cells, and that the antioxidant N-acetylcysteine ​​can inhibit these changes.