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目的研究沙苑子黄酮(flavonoids of Astragali complanali,FAC)的抗辐射作用。方法雌性昆明小鼠随机分为正常组、模型组、阳性对照组(利血生组)、FAC-1组、FAC-2组、FAC-3组等6组,分别给予相应的药物干预。第7天除正常对照组外各组小鼠均接受6 GY60Coγ射线一次性全身照射。对各组小鼠肝脏HE染色切片观察FAC对辐射损伤的保护效应。采用试剂盒测定小鼠肝脏匀浆中超氧化物歧化酶(SOD)水平、谷胱甘肽过氧化物酶(GSH-Px)抗氧化酶的活力、脂质过氧化产物丙二醛(MDA)的含量和总抗氧化能力(T-AOC)的变化情况。采用分光光度法测定H2O2诱导的红细胞溶血、Fe2+-Vit C诱导的肝线粒体肿胀度以及O2-,.OH和DPPH变化情况。结果与正常组比较,辐射能够引起小鼠血象指标及生理结构的显著改变(P<0.05,P<0.01);氧化能够在体外引起自由基和溶血及线粒体肿胀的发生(P<0.01)。与模型组比较,FAC增强了SOD、GSH-Px活力,降低MDA的含量(P<0.01);高剂量FAC的作用普遍强于阳性对照药(P<0.05)。FAC有很强的清除自由基的作用,对3种自由基的清除率都达到60%以上,其中对DPPH的清除率达到90%,高剂量FAC的作用要强于阳性对照组(P<0.05,P<0.01)。与模型组比较,FAC抑制线粒体肿胀和红细胞溶血的作用为50%(P<0.01);高剂量FAC的作用与阳性对照药相似。HE切片显示FAC能显著减轻辐射对小鼠肝脏造成的坏死、肿胀、炎症浸润。各FAC组之间有随着给药剂量增加而作用增加的剂量依赖关系(P<0.05,P<0.01)。结论 FAC对辐射小鼠有保护作用,其作用机制与提高抗氧化能力、保护细胞及组织的正常形态和功能有关。
Aim To study the anti-radiation effect of flavonoids of Astragali complanali (FAC). Methods Female Kunming mice were randomly divided into 6 groups: normal group, model group, positive control group (reserpine group), FAC-1 group, FAC-2 group and FAC-3 group. On the 7th day except for the normal control group, each group of mice received a one-time whole body irradiation with 6 GY60Coγ ray. The liver of each group was stained with HE to observe the protective effect of FAC on radiation injury. The levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) antioxidant enzyme activity, lipid peroxidation product malondialdehyde (MDA) Content and total antioxidant capacity (T-AOC) changes. H2O2-induced erythrocyte hemolysis, mitochondria swelling induced by Fe2 + -Vit C and changes of O2 -, OH and DPPH were measured by spectrophotometry. Results Compared with the normal group, the radiation induced a significant change in the blood parameters and the physiological structure of the mice (P <0.05, P <0.01). The oxidation induced the swelling of free radicals, hemolysis and mitochondria in vitro (P <0.01). Compared with the model group, FAC enhanced the activity of SOD and GSH-Px and decreased the content of MDA (P <0.01). The effect of high dose FAC was stronger than that of positive control (P <0.05). FAC has a strong ability of scavenging free radicals, and the clearance rate of all three free radicals is above 60%. The clearance rate of DPPH reaches 90%, and the effect of high dose FAC is stronger than that of the positive control (P <0.05, P <0.01). Compared with the model group, the effect of FAC on mitochondrial swelling and erythrocyte hemolysis was 50% (P <0.01). The effect of high-dose FAC was similar to that of positive control. HE sections showed that FAC can significantly reduce the necrosis, swelling and inflammatory infiltration caused by radiation on the liver of mice. There was a dose-dependent increase in the dose-dependent increase in each FAC group (P <0.05, P <0.01). Conclusion FAC has a protective effect on irradiated mice, and its mechanism of action is related to the enhancement of antioxidant capacity and protection of the normal morphology and function of cells and tissues.