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目的:探讨食管鳞癌组织差异基因表达。方法:分别取3例无食管鳞癌家族史患者的癌及癌旁组织(Ⅰ组)和6例有食管鳞癌家族史患者的癌及癌旁组织(Ⅱ组)等量混合,抽提RNA,将RNA逆转录合成相应cDNA,以Cy5和Cy3标记cDNA作为探针,在含有14 000点人类基因组芯片(BiostarH-140s)上进行杂交。用scanarray 4000扫描仪扫描芯片荧光信号图像,用GenePix Pro 3.0软件对扫描图像进行数字化处理和分析。结果:依Ratio(Cy5/Cy3)>2.0或<0.5的数据项为差异表达的基因,Ⅰ组和Ⅱ组分别为1 855个和613个。两组具相同异常表达的基因有60个,含表达序列标签(EST)30个。30个基因中,除上皮细胞膜蛋白-1、骨桥素、人类泛素耦联酶E2C、成视网膜细胞瘤样-2共4个基因外,其余26个基因中未见与食管癌相关的报道。结论:利用基因芯片法可高通量地筛选出散发和有食管鳞癌家族史患者癌组织中共同存在的异常表达的基因谱,为进一步阐明食管癌的发病机制提供信息。
Objective: To investigate the differential gene expression in esophageal squamous cell carcinoma tissue. Methods: The cancer and para-cancerous tissues (group Ⅰ) and the cancer and paracancerous tissues (group Ⅱ) of 3 patients with family history of esophageal squamous cell carcinoma and 6 patients with family history of esophageal squamous cell carcinoma , RNA was reverse transcribed to the corresponding cDNA, Cy5 and Cy3-labeled cDNA was used as a probe and hybridization was performed on a 14,000-point human genomic chip (Biostar H-140s). The fluorescence signal of the chip was scanned with scanarray 4000 scanner and the scanned image was digitized and analyzed with GenePix Pro 3.0 software. RESULTS: Data items with Ratio (Cy5 / Cy3)> 2.0 or <0.5 were differentially expressed genes, with 1 855 and 613 for group I and group II, respectively. There were 60 genes with the same abnormal expression in both groups, including 30 expressed sequence tags (ESTs). Among the 30 genes, no esophageal cancer was reported in 26 of the 26 genes besides epithelial membrane protein-1, osteopontin, human ubiquitin-conjugating enzyme E2C and retinoblastoma-2 . CONCLUSION: The gene chip method can be used to screen out the abnormal gene expression profiles coexisting in cancerous tissues of esophageal squamous cell carcinoma with high-throughput screening and to provide information for further elucidating the pathogenesis of esophageal cancer.