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目的:探讨中亚苦蒿硅胶柱分离组分（AEM-SC）对小鼠树突状细胞（DC）成熟及免疫功能的影响。方法:制备中亚苦蒿大孔吸附树脂70%乙醇洗脱组分，经硅胶柱进一步分离得到洗脱组分AEM-SC，并对其多糖、总黄酮、三萜类含量进行测定。流式细胞术检测AEM-SC对DC表面分子表达水平、抗原吞噬能力及刺激同种异体T细胞增殖能力的影响；酶联免疫吸附测定（ELISA）法检测AEM-SC对DC细胞因子表达的影响。结果:AEM-SC中多糖、黄酮类和萜类的含量分别为10.12%、5.70%和3.62%。体外功能实验结果显示，AEM-SC可明显降低脂多糖诱导的DC表面分子CD40、CD86和主要组织相容性复合体Ⅱ类（MHC-Ⅱ）的表达以及细胞因子白细胞介素-12p40（IL-12p40）、肿瘤坏死因子α（TNF-α）和IL-6的水平（均n P<0.05），提高DC摄取抗原的能力（n P<0.01），降低DC刺激小鼠脾脏CD4n + T与CD8n + T淋巴细胞增殖的能力（均n P<0.001）。小鼠炎症模型实验中，AEM-SC可明显降低脂多糖诱导的小鼠体内DC表面分子CD40、CD86、CD80的表达（均n P<0.001）以及血清中细胞因子TNF-α、IL-12p40的表达（均n P<0.01）。n 结论:AEM-SC在体外与体内实验中均表现出抑制DC成熟的能力，显示AEM-SC具有一定的免疫抑制作用。“,”Objective:To investigate the effect of silica gel column separation component of n Artemisia asiatica (AEM-SC) on the maturation and immune function of mouse dendritic cells (DCs).n Methods:Artemisia asiatica components were prepared by macroporous resin eluted with 70% ethanol, and then isolated by silica gel column to obtain AEM-SC. The contents of polysaccharides, flavonoids and triterpenes were quantified. Flow cytometry was used to detect the expression level of DCs surface molecules and antigen phagocytosis ability and to stimulate the proliferation of allogeneic T cells. ELISA method was used to detect the effect of DCs on cytokine secretion.n Results:The contents of polysaccharides, flavonoids and triterpenes in AEM-SC were 10.12%, 5.7% and 3.62%, respectively. Functional tests showed that AEM-SC significantly reduced the expression levels of LPS-induced DCs surface molecules CD40, CD86 and MHC-II, reduced the expression levels of inflammatory cytokines IL-12p40, TNF-α and IL-6 (alln P<0.05), improve the ability of phygocytosis (n P<0.01), and reduce the ability of DCs to stimulate the proliferation of CD4n +T and CD8n +T lymphocytes in the spleen of mice (all n P<0.001). In the inflammatory mouse model experiment, AEM-SC significantly reduced the expression levels of DCs surface molecules CD40, CD86, CD80 (alln P<0.001), and the expression levels of inflammatory cytokines TNF-α, IL-12p40 in serum (alln P<0.01).n Conclusions:AEM-SC can inhibit the maturation of DCs-induced LPS both n in vitro and n in vivo, indicating that AEM-SC has the immunosuppressive effect.n