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目的了解实习护士洗手状况及影响因素，探讨防范措施，降低医院的感染率。方法采取自行设计的调查问卷对湖北省某医院98名实习护士进行调查，对调查结果进行统计分析。结果15.31%的实习护士操作前经常洗手，17.35%的实习护士脱手套后经常洗手，13.27%的实习护士接触患者前经常洗手等。结论大部分的实习护士洗手状况差。医院管理者应对实习护士进行手卫生的教育干预，加强带教管理，实施手卫生的监测，完善医院洗手设备配置，提高手卫生依从性，从而降低医院感染率。“,”Objective To survey the status of the practice nurses to wash their hands and its influence factors, discuss preventive measures, reduce the hospital infection rate. Methods Take the self-designed questionnaire on 98 practice nurses from a hospital in Hubei province in the form of questionnaire survey, statistical analysis of survey results. Results 15.31% of the practice nurse often wash your hands before operation, 17.35% of the practice nurse after set to sel to wash your hands often, 13.27% of the practice nurse often wash your hands before contact with the patient, etc. Conclusion Most of the practice nurse hand washing is in poor conditions. The nurse hand hygiene education intervention, strengthen teaching management, monitoring the implementation of hand hygiene, improve the hospital hand washing equipment, improve the hand hygiene compliance behavior, standardized management.