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朊毒体是传染性海绵样脑病的病原,主要由糖蛋白PrP~(Sc)组成,PrP~(Sc)由正常细胞表面蛋白PrP~C异构而成。从感染朊毒体到出现临床症状,极微量的PrP~(Sc)即可将宿主PrP~C不断转变为PrP~(Sc),最后导致患者大脑中大量PrP~(Sc)聚集。目前众多检测方法均因非脑组织中PrP~(Sc)含量过少而受限,以致延误诊治。本文作者报道通过循环扩增误折叠蛋白,大量PrP~C在微量 PrP~(Sc)存在下转变为PrP~(Sc)类似物(PrPres),从而能在非脑组织中早期检出微量PrP~(Sc)。具体方法为:①脑组织匀浆的制备:将对照大鼠及感染了瘙痒
Prion is the causative agent of transmissible spongiform encephalopathies, which is mainly composed of the glycoprotein PrP ~ (Sc), which is isomerized from the normal cell surface protein PrP ~ C. PrP ~ (Sc) can be transformed into PrP ~ (Sc) by a small amount of PrP ~ (Sc) from the prion to clinical symptoms. Finally, a large amount of PrP ~ (Sc) is accumulated in the brain. At present, many detection methods are limited due to the small amount of PrP (Sc) in non-brain tissue, resulting in delayed diagnosis and treatment. The authors report that a large amount of PrP-C is converted to PrP ~ (Sc) analogue (PrPres) in the presence of a small amount of PrP ~ (Sc) by cyclic amplification of misfolded proteins, so that a small amount of PrP ~ (Sc). Specific methods: ① preparation of brain homogenates: the control rats and infected with itching