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目的了解早期气道接触细菌抗原对辅助性T淋巴细胞1/2(Th1/Th2)平衡以及大鼠成年后哮喘气道炎症的影响,研究哮喘的发病机制。方法将新生SD大鼠随机分为3个细菌抗原组、哮喘组及对照组,细菌抗原组大鼠分别于出生后第1、3、5、7 d鼻咽部给予107、106、105灭活肺炎链球菌10μL,哮喘组及对照组大鼠则给予磷酸盐缓冲液(PBS)10μL;大鼠饲养至成年后造模,观察各组大鼠肺组织病理、支气管肺泡灌洗液(BALF)白细胞分类、血清中白细胞介素-4(IL-4)、γ-干扰素(IFN-γ)、卵蛋白-IgE含量。结果肺组织病理显示,早期接触灭活细菌的大鼠气道炎症较重;107、106抗原组大鼠BALF中淋巴细胞百分比为(32.94±13.62)%、(29.50±8.92)%,与哮喘组比较明显增多(P<0.05);107抗原组大鼠血清中卵蛋白-IgE水平(0.33±0.07)较哮喘组明显升高(P<0.01),IFN-γ水平为(11.93±2.74)pg/mL,较哮喘组明显下降(P<0.05)。结论早期气道接触细菌抗原可加重Th1/Th2失衡以及大鼠成年后哮喘气道炎症。
Objective To investigate the effects of early airway bacterial contact on the balance of Th1 / Th2 and the airway inflammation in asthmatic rats, and to study the pathogenesis of asthma. Methods Newborn SD rats were randomly divided into three groups: bacterial antigen group, asthma group and control group. The rats in bacterial antigen group were given 107,106,105 inactivated nasopharynx on the 1st, 3rd, 5th and 7th days after birth respectively Streptococcus pneumoniae 10μL, asthma group and control group rats were given phosphate buffer solution (PBS) 10μL; rats were fed to adult model, observe the pathological changes of lung tissue, bronchoalveolar lavage fluid (BALF) leukocytes Classification, serum interleukin-4 (IL-4), interferon-γ (IFN-γ), egg protein-IgE content. Results The histopathology of lung showed that the airway inflammation of rats exposed to inactivated bacteria was heavier in the early stage. The percentage of lymphocytes in the BALF of 107,106 antigen group was (32.94 ± 13.62)%, (29.50 ± 8.92)%, (P <0.01), and the level of IFN-γ was (11.93 ± 2.74) pg / ml in 107 antigen group (0.33 ± 0.07) mL, significantly lower than the asthma group (P <0.05). Conclusions Early airway exposure to bacterial antigens may aggravate the imbalance of Th1 / Th2 and asthma airway inflammation in rats.