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玉米内生细菌YY1菌株为枯草芽孢杆菌,对玉米大斑病菌(Setosphaeria turcica)有强烈的拮抗作用,通过产生抗菌物质发挥抑菌活性。以培养基的无菌滤液对玉米大斑病菌的拮抗活性为检测指标,确定其产生抗菌物质所需要的最佳碳源、氮源及无机盐,通过正交试验得到该菌株产生抗菌物质的培养基配方,并对其发酵工艺条件进行优化。结果表明,最佳培养基为可溶性淀粉3.0%,蛋白胨2.0%,KH2PO40.02%,MnCl20.01%。发酵工艺参数优化结果为培养基装液量30 mL/250 mL,接种量4%,培养基初始pH值为9.0,28℃条件下、120 r/min摇床振荡培养48 h。经发酵条件优化后,其抗菌活性有显著提高。
The endophyte YY1 strain of corn is Bacillus subtilis and has a strong antagonistic effect on Setosphaeria turcica, and exerts antibacterial activity through the production of antibacterial substances. Antagonistic activity of the aseptic filtrate of the culture medium on S. turcica was used as the detection index to determine the optimal carbon source, nitrogen source and inorganic salt needed for producing the antibacterial substance. The culture of the strain producing the antibacterial substance was obtained by orthogonal test Based formula, and optimize the fermentation process conditions. The results showed that the optimum medium was soluble starch 3.0%, peptone 2.0%, KH2PO40.02% and MnCl20.01%. The optimized fermentation parameters were as follows: 30 mL / 250 mL of medium, 4% of inoculum, initial pH value of medium 9.0, shaking culture at 120 r / min for 48 h at 28 ℃. Optimized fermentation conditions, the antibacterial activity was significantly improved.