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本文作者对彭亨丝虫、犬恶丝虫微丝蚴在4种细胞系及另外两种培养系中的发育进行了研究。作者用无菌操作自感染猫和犬的血液中获得彭亨丝虫、犬恶丝虫的微丝蚴,以5微米孔径的薄膜过滤,将微丝蚴与红细胞分离,然后悬浮在昆虫组织培养基内,或 pH 7. 4的Hank 平衡盐溶液内备用。所用培养方法有三:第1种方法包括将分离出来的微丝蚴接种在4种蚊虫细胞系培养基内(马来伊蚊系MM/MK、假鳞斑伊蚊系 MM/VP_(12) 、埃及伊
The authors investigated the development of penicillium and penicillium filariasis in four cell lines and two other lines. The authors obtained a microflora of penicilli filari and canine filariasis from a bloodstream of infected cats and dogs with aseptic procedures, filtered through a 5 micron pore membrane, separated microfilariae from erythrocytes, and suspended in insect tissue culture medium , Or pH 7.4 Hank balanced salt solution for use. There are three culture methods used: The first method consists of inoculation of the isolated microfilariae in four mosquito cell line medium (Malayan mosquito line MM / MK, P. pseudoseratus MM / VP 12, Egypt Iraq