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Gas chromatography-mass spectrometry(GC-MS)and the chemometric resolution method(altative moving window factor analysis,AMWFA)were used for comparative analysis of volatile constituents in herbal pair(HP)flos lonicerae-caulis lonicerae(FL-CL)and its single herbs.The temperature-programmed retention index(PTRI)was also employed for the identification of compounds.In total,44,39,and 50 volatile chemical components in volatilc oil of FL,CL and HP FL-CL were separately determined qualitatively and quantitatively,accounting for 87.22%,94.54% and 90.08% total contents of volatile oil of FL,CL and HP FL-CL,respectively.The results show that there are 32 common volatile constituents between HP FL-CL and single herb FL,33 common volatile constituents between HP FL-CL and single herb CL,and 10 new constituents in the volatile oil of HP FL-CL.