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目的通过对多种消毒方式进行比较研究,对实验动物屏障系统消毒方式的选择提供参考依据。方法选取不同测定时间点(消毒前及消毒后的30 min,2h、6 h、24 h、48 h、72 h),检查消毒完成前后实验室的沉降菌最大平均浓度,比较各消毒方式最佳效果和持续时间;通过消毒剂在特定条件下对绿脓杆菌、金黄色葡萄球菌进行杀灭实验,比较消毒剂的杀菌率;以及各种消毒方式的优缺点。结果两种用甲醛的消毒方式效果最好;臭氧、过氧乙酸最佳消毒效果持续时间保持在48 h内;消毒泡腾片及84Ⅱ消毒液最佳消毒效果持续时间保持在24 h内;新洁尔灭消毒后沉降菌最大平均浓度未符合相关国家标准的规定,且沉降菌最大平均浓度增长速度较快。各消毒剂对两种实验菌株的杀菌率均在99%以上,但新洁尔灭对绿脓杆菌杀菌效果差。结论甲醛适用于屏障系统启用前及可疑传染时的消毒,臭氧、过氧乙酸适用于房间的整体消毒,消毒泡腾片、84Ⅱ消毒液、新洁尔灭适用于实验动物室维持常规消毒。
Objective To compare the different disinfection methods and provide references for the selection of disinfection methods for laboratory animal barrier systems. Methods The maximum average concentration of sedimentation in laboratory before and after disinfection was checked at different time points (before disinfection and after disinfection for 30 min, 2 h, 6 h, 24 h, 48 h, 72 h). The best disinfection methods were compared Effect and duration; disinfectant under specific conditions of Pseudomonas aeruginosa, Staphylococcus aureus killing experiments to compare the disinfectant sterilization rate; and the advantages and disadvantages of various disinfection methods. The results showed that the two methods of formaldehyde disinfection were the best. The best disinfection effect of ozone and peracetic acid was maintained within 48 h. The best disinfection efficiency of disinfecting effervescent effervescent tablets and 84 Ⅱ disinfectant was within 24 h. After disinfection the maximum average concentration of sedimentation did not meet the relevant national standards, and the maximum average concentration of sedimentation increased rapidly. Bactericidal rates of the two disinfectants on the two experimental strains were above 99%, but the bactericidal effect of benzalkonium bromide on Pseudomonas aeruginosa was poor. Conclusions Formaldehyde is suitable for the disinfection of barrier system before and during suspicious infection. Ozone and peracetic acid are suitable for the overall disinfection of the room, disinfection effervescent tablets, 84Ⅱ disinfectant and benzalkonium bromide for routine disinfection of laboratory animals.