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目的:了解山茶籽联合雌二醇对去卵巢大鼠骨组织和骨代谢指标的影响,为中西医结合治疗绝经后骨质疏松症提供实验依据。方法:6月龄健康雌性大白鼠120只,分成5个实验组各24只:即假手术组(sham)、去卵巢模型组(OVX)、山茶籽组(Ts)、雌二醇组(E2)、小剂量山茶籽醇提取物+雌二醇组(Ts+E2)。各实验组在第4、8、12和20周随机取6只大鼠取股骨切片观察骨组织,采血测量血钙、磷和碱性磷酸酶,数据进行统计学分析。结果:OVX组的第4、8周血钙、磷明显低于sham组(P<0.01),但OVX组的第12、20周血钙、磷与sham组相比差异无统计学意义(P>0.05)。OVX组的第4、8、12、20周血碱性磷酸酶均明显高于sham组(P<0.01)。3个治疗组各时间的血钙、磷和碱性磷酸酶与sham组差异无统计学意义(P>0.05)。小剂量的雌二醇联合山茶籽能使去卵巢大鼠骨组织和血钙、磷和血碱性磷酸酶基本恢复正常(P>0.05),与较大剂量的山茶籽组或较大剂量的雌二醇组相比无明显差异(P>0.05)。结论:山茶籽醇提取物对去卵巢大鼠的骨质疏松症的治疗效果良好;较小剂量的雌二醇与山茶籽对去卵巢大鼠的骨质疏松症的治疗效果与单独使用较大剂量的山茶籽或较大剂量的雌二醇相比治疗效果相似。
Objective: To understand the effect of camellia seed combined with estradiol on bone tissue and bone metabolism in ovariectomized rats, and to provide experimental evidence for the treatment of postmenopausal osteoporosis by integrated traditional Chinese and western medicine. Methods: A total of 120 healthy 6-month-old female rats were randomly divided into five experimental groups: sham, ovariectomized group (OVX), camellia seed group (Ts) and estradiol group ), Small dose of Camellia oleifera extract + estradiol group (Ts + E2). At the 4th, 8th, 12th and 20th weeks of each experimental group, 6 rats were randomly selected for the observation of bone tissue, blood calcium, phosphorus and alkaline phosphatase were measured and the data were statistically analyzed. Results: The levels of serum calcium and phosphorus in the OVX group were significantly lower than those in the sham group at the 4th and 8th weeks (P <0.01), but there was no significant difference between the sham group and the 12th and 20th weeks in the OVX group (P > 0.05). The serum ALP levels at 4, 8, 12 and 20 weeks in OVX group were significantly higher than those in sham group (P <0.01). There was no significant difference in serum calcium, phosphorus and alkaline phosphatase between the three treatment groups and the sham group (P> 0.05). Small doses of estradiol combined with camellia seed could return normal bone tissue, serum calcium, phosphorus and ALP in ovariectomized rats (P> 0.05), and higher doses of camellia seed or higher dose of Estradiol group no significant difference (P> 0.05). Conclusion: Camellia oleifera alcohol extract is effective in the treatment of osteoporosis in ovariectomized rats. The effect of small doses of estradiol and camellia sinensis on osteoporosis in ovariectomized rats is better than that of single use Therapeutic efficacy was similar for doses of camellia or larger doses of estradiol.