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为纯化和鉴定感觉神经特异蛋白，以兔脊髓背根神经节及背根纤维组织为材料，通过制备匀浆、离子交换层析 Ｄ Ｅ Ａ Ｅ Ｓｅｐｈａｃｅｌ，高压液相凝胶过滤层析分离纯化了脊神经感觉神经元 ３５ ｋ Ｄ蛋白，将其作为抗原制备抗 ３５ ｋ Ｄ 多克隆抗体． Ｗ ｅｓｔｅｒｎ ｂｌｏｔ 的结果表明，该蛋白特异地存在于脊感觉神经而不存在于脊运动神经．并初步观察到它对鸡胚背根节有神经营养作用．
In order to purify and identify the sensory nerve specific protein, the spinal cord dorsal root ganglion and dorsal root ganglion were harvested and purified by preparative homogenization, ion exchange chromatography (DEAE Sephacel) and high pressure liquid gel filtration chromatography The 35 kD protein of spinal sensory neurons was prepared as an anti-35 kD polyclonal antibody. Western blot results show that the protein is specifically present in the spinal sensory nerve rather than in the spinal motor nerve. And initially observed that it has neurotrophic effects on chicken embryo.