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肉孢子虫可致肉孢子虫病,是一种人畜共患寄生虫病。本文报告梭状肉孢子虫的扫描电镜观察结果。 一、材料和方法 取新鲜水牛食道肌和腰肌,仔细剥离出梭状肉孢子虫包囊,取部分带有肌肉的包囊,经生理盐水清洗后,立即用1%锇酸固定1h,双蒸水清洗后,采用25%dDMSO(二甲基亚砜)、50％DMSO先后处理各20min,继而将标本冷冻断裂,再用1%锇酸固定1h。双蒸水清洗后,0.1%锇酸软化72h,双蒸水清洗。用2%单磷酸处理12h。清洗后,1%饿酸固定、清洗、系列酒精脱水(其
Sarcocystosis can cause sarcocystosis, is a zoonotic parasitic disease. This article reports the scanning electron microscopy results of Schizosacci. First, the material and methods Take fresh buffalo esophageal muscle and psoas muscle, carefully stripped of Schistosoma sp. Cysts, take part of the capsule with muscle, after saline wash, immediately fixed with 1% osmium tetroxide 1h, double After washing with distilled water, the samples were treated with 25% dDMSO (dimethylsulfoxide) and 50% DMSO for 20 min each, followed by freeze-thawing the samples and fixing with 1% osmium tetroxide for 1 h. Double distilled water, 0.1% osmium teurate softened 72h, double distilled water. Treated with 2% monophosphate for 12h. After cleaning, 1% hungry acid fixed, cleaned, the series of alcohol dehydration (its