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旨在研究杂交谷子品种‘张杂谷3号’对干旱的生理响应特征及其抗旱性强度,为拓展中国旱作区农业发展途径及杂交谷子在旱作农业生产体系中的推广提供支持。利用不同浓度PEG-6000溶液模拟干旱胁迫,比较了‘张杂谷3号’、亲本1、亲本2和常规种‘8311’共4种谷子幼苗叶片叶绿素、游离脯氨酸、丙二醛(MDA)和超氧化物歧化酶(SOD)等生理生化指标对不同程度模拟干旱的响应特征。不同浓度PEG渗透胁迫的模拟干旱导致4种谷子材料幼苗叶片的叶绿素含量均明显下降,‘张杂谷3号’降幅最小;不同胁迫处理下4种幼苗叶片的游离脯氨酸随胁迫程度急剧上升;不同胁迫下,4种材料幼苗叶片内的MDA含量均有不同程度的增加,其中‘张杂谷3号’增幅最小;4种幼苗的SOD活性随胁迫加剧呈上升趋势,20%和30%PEG胁迫下,SOD活性均快速上升,其中‘张杂谷3号’上升幅度最大。4种材料在干旱胁迫下的适应能力存在差异,‘张杂谷3号’的耐旱性最强,常规谷子‘8311’次之,亲本2最差。
The purpose is to study the physiological responses to drought and the drought resistance of ’Zhangzigu No.3’ hybrid millet variety, and to provide support for expanding agricultural development in dryland areas of China and popularizing millet millet in dry farming system. Different concentrations of PEG-6000 solution were used to simulate drought stress. Chlorophyll content, free proline content, malondialdehyde (MDA) content in leaves of 4 millet seedlings of ’Zhangzigu No.3’, ’Parents 1, 2’ and ’8311’ ) And superoxide dismutase (SOD) and other physiological and biochemical indicators of response to varying degrees of simulated drought response. Simulated drought caused by different concentrations of PEG osmotic stress resulted in significant decrease of chlorophyll content in leaves of the four kinds of millet materials and the smallest decrease of ’Zhangzigu No.3’. The free proline content in leaves of four seedlings increased sharply with different stresses Under different stresses, the content of MDA in seedling leaves increased with different degrees, of which, the increase of ’ZZG3’ was the smallest; the activity of SOD of four seedlings increased with the increase of stress, while that of 20% and 30% Under the stress of PEG, SOD activity increased rapidly, of which ’Zhang Zha Gu No.3’ had the largest increase. The adaptability of the four materials under drought stress varied. “Zhang Zha Gu No.3” had the strongest drought tolerance, the second was “normal” and the second was the worst.