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目的研究ITP患儿淋巴细胞亚群、血小板抗体及骨髓象在发病期的变化。方法应用流式细胞术(FCM)检测34例ITP患儿发病期外周血淋巴细胞亚群的水平(CD3+、CD4+、CD8+、CD19+、CD4+/CD8+、NK)。用ELISA法检测血小板表面血小板相关抗体IgG、IgM、IgA。同时抽取患儿骨髓制作骨髓片。结果ITP患儿发病期组CD4+/CD8+的比例显著降低,NK细胞显著降低,CD19+显著增高,ITP患儿组血小板抗体PAIgG、PAIgM和正常对照组比较显著增高(P<0.01),同时CD19+增高与血小抗体PAIgG、PAIgM增高有明显相关性(P<0.001)。骨髓象中巨核细胞总数和对照组比较显著增高(P<0.01),同时原幼巨核细胞总数、颗粒巨核细胞总数明显增高,而产板型巨核细胞总数明显降低。结论ITP患儿存在细胞免疫和体液免疫紊乱。
Objective To study the changes of lymphocyte subsets, platelet antibodies and bone marrow in ITP children during the onset period. Methods The levels of peripheral blood lymphocyte subsets (CD3 +, CD4 +, CD8 +, CD19 +, CD4 + / CD8 +, NK) in 34 patients with ITP were detected by flow cytometry (FCM). ELISA platelet surface platelet-related antibodies IgG, IgM, IgA. At the same time extraction of bone marrow in children with bone marrow tablets. Results The proportion of CD4 + / CD8 + cells in ITP group was significantly lower than that in control group (P <0.01), and the level of CD19 + and CD19 + in ITP group was significantly higher than that in ITP group (P <0.01) Antibodies to PAIgG and PAIgM were significantly correlated (P <0.001). The total number of megakaryocytes in bone marrow was significantly higher than that in the control group (P <0.01). At the same time, the total number of megakaryocytes and the total number of megakaryocytes were significantly increased, while the total number of plate-producing megakaryocytes was significantly decreased. Conclusion ITP children have cellular and humoral immune disorders.