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目的研究肺癌组织中脆性组氨酸三位体（ＦＨＩＴ）基因的改变。方法采用逆转录聚合酶链反应（ＲＴＰＣＲ）和逆转录聚合酶链反应单链构象多态性（ＲＴＰＣＲＳＳＣＰ）方法分析４７例肺癌组织和其中１６例相应的转移性肺门淋巴结。结果６８％（３２／４７）肺癌原发灶（包括２例肺鳞状细胞原位癌）和９４％（１５／１６）转移性肺门淋巴结出现ＦＨＩＴ转录本缺失，二者统计学差异有显著性（Ｐ＜０．０５）。ＦＨＩＴ基因转录本缺失主要发生于编码区。ＲＴＰＣＲＳＳＣＰ分析未发现突变。结论（１）在肺癌组织中ＦＨＩＴ基因转录本缺失是频发事件，且可能为早期事件；（２）ＦＨＩＴ基因突变可能是少见事件；（３）转移性肺门淋巴结ＦＨＩＴ基因转录本缺失频率与肺癌原发灶相比差异有显著性，提示具有ＦＨＩＴ转录本缺失的肺癌细胞具有更明显的恶性表型和恶性行为
Objective To study the alteration of fragile histidine triad (FHIT) gene in lung cancer tissues. Methods RT-PCR and reverse transcriptase polymerase chain reaction single-strand conformation polymorphism (RT-PCR-SSCP) were used to analyze 47 cases of lung cancer and 16 cases of metastatic disease. Hilar lymph nodes. Results FHIT transcripts were absent in 68% (32/47) primary lung cancer (including 2 lung squamous cell carcinomas in situ) and 94% (15/16) metastatic hilar lymph nodes. Sex (P<0.05). Loss of FHIT gene transcripts occurs mainly in the coding region. RT-PCR-SSCP analysis found no mutations. Conclusion (1) FHIT gene transcript deletion is a frequent event in lung cancer and may be an early event; (2) FHIT gene mutation may be a rare event; (3) frequency of FHIT gene transcript deletion in metastatic hilar lymph node The difference of lung cancer primary lesions was significant, suggesting that lung cancer cells with loss of FHIT transcript have more obvious malignant phenotype and malignant behavior