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目的:评价胡黄连苷元对大鼠“两次打击”损伤的保护作用.方法:采用大鼠“两次打击”损伤模型(失血性休克40 mmHg 45 min后iv细菌内毒素LPS 150 μg/kg).大鼠随机分为七组:对照组,内毒素组,失血组,失血/内毒素组和失血/内毒素+胡黄连苷元(2.5,5.0,10.0 mg/kg)组.胡黄连苷元溶于复苏液(生理盐水,NS)中并在2 h内经静脉给药。在LPS或NS注射后,观察大鼠8,16,24及48 h存活率;检测肺组织(iv LPS后3 h及6 h)及血清(失血前,失血后及iv LPS/NS后0,0.5,1,2,4,6 h)丙二醛含量;检测肺及肝组织(iv LPS/NS后 3 h及 6 h)髓过氧化物酶活性.结果:与对照组比较,“两次打击”损伤大鼠8 h(64.3％,P<0.05),16 h(35.7％,P<0.01),24 h(28.6％,P<0.01),48 h(14.3％,P<0.01)存活率显著降低;血清及肺组织丙二醛含量显著增多,肺、肝组织髓过氧化物酶活力显著升高.胡黄连苷元剂量依赖性升高失血性休克大鼠平均动脉压(P<0.05),提高大鼠存活率,降低血清及组织丙二醛含量,下调肺及肝组织髓过氧化物酶活力.结论:胡黄连苷元可保护由失血性休克及细菌内毒素诱导的大鼠“两次打击”损伤.
OBJECTIVE: To evaluate the protective effect of picroside aganist against “twice-hit” injury in rats.Methods: The rat model of “twice-hit” injury was established after 40 min of hemorrhagic shock (LPS 150 μg / kg iv ) Were randomly divided into seven groups: control group, endotoxin group, blood loss group, blood loss / endotoxin group and blood loss / endotoxin + picrotisidein group (2.5, 5.0, 10.0 mg / The element was dissolved in resuscitation fluid (NS, NS) and administered intravenously within 2 h. After LPS or NS injection, the survival rate of rats at 8, 16, 24 and 48 h was observed. The lung tissue (3 h and 6 h after iv LPS) and serum (pre-blood loss, 0.5, 1, 2, 4 and 6 h), and the activity of myeloperoxidase in lung and liver (3 h and 6 h after iv LPS / NS) were measured.Results: Compared with the control group, The rats were sacrificed for 8 h (64.3%, P <0.05), 16 h (35.7%, P <0.01), 24 h (P <0.05) .Compared with the control group, the content of malondialdehyde in serum and lung tissue increased significantly, and the activity of myeloperoxidase in lung and liver tissue increased significantly.Peptide increased the average arterial pressure in hemorrhagic shock rats in a dose-dependent manner (P <0.05) , Improve the survival rate of rats, reduce the serum and tissue MDA content and down-regulate the activity of myeloperoxidase in lung and liver tissue.Conclusion: Picroside can protect hemorrhagic shock and bacterial endotoxin-induced rat “two Times hit ”damage.