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从9例HBsAg(RPHA法)和HBeAg(ID法)均阳性的母亲流产的9例胎儿肝组织中,抽提高分子量肝细胞DNA,与~(32)P标记的克隆HBV DNA探针进行Southern blot杂交试验,以检测胎肝细胞中HBV
Nine cases of fetal liver tissue from 9 mothers with positive HBsAg (RPHA) and HBeAg (ID) positive mothers were harvested for DNA extraction from high molecular weight hepatocytes and Southern blot with a ~ (32) P labeled clone HBV DNA probe Hybridization assay to detect HBV in fetal hepatocytes