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目的:观察二氢石蒜碱(dihydrolycorine,DL)对大鼠局部脑缺血/再灌注损伤的治疗作用和可能机制。方法:线栓法制备右侧大脑中动脉局灶性脑缺血模型,缺血1 h再灌注24 h。SD大鼠随机分为假手术组、模型组和DL低、中、高剂量组,于大鼠脑缺血后即刻及复灌后11 h及23 h DL组腹腔注射DL(10,20,40 mg.kg-1)治疗,假手术组和模型组则注射生理盐水对照。观察不同剂量DL对脑缺血再灌注大鼠神经功能状态、脑梗塞面积、脑水肿程度和脑组织髓过氧化物酶(myeloperoxidase,MPO)活性的影响。结果:脑缺血后给予DL可明显降低缺血侧脑梗死及脑水肿程度,使神经病学症状得到明显改善,降低缺血再灌注损伤后脑组织内MPO的活性(P<0.05)。结论:DL对脑缺血/再灌注损伤具有治疗作用,可能与其减轻脑缺血再灌注所致脑组织的炎症反应有关。
Objective: To observe the therapeutic effect and possible mechanism of dihydrolycorine (DL) on focal cerebral ischemia / reperfusion injury in rats. Methods: The right middle cerebral artery occlusion (MCAO) model was established by thread occlusion. SD rats were randomly divided into sham operation group, model group and DL low, medium and high dose groups. The rats in DL group were injected intraperitoneally with DL (10, 20, 40) at 11 h and 23 h after cerebral ischemia mg.kg-1), sham operation group and model group were injected saline control. To observe the effects of DL at different doses on neurological status, infarct size, degree of brain edema and activity of myeloperoxidase (MPO) in cerebral ischemia-reperfusion rats. Results: Administration of DL after cerebral ischemia reduced the severity of ischemic cerebral infarction and cerebral edema remarkably, and significantly improved the neurological symptoms and decreased the activity of MPO in the brain tissue after ischemia / reperfusion (P <0.05). CONCLUSION: DL has a therapeutic effect on cerebral ischemia / reperfusion injury, which may be related to reducing the inflammatory reaction of brain tissue induced by cerebral ischemia-reperfusion.