鸡胚或细胞培养分离流感病毒在流感暴发的流行病学调查和疫苗的研制中非常重要。细胞培养病毒的方法在分离引起1997年香港流感流行的H5N1禽流感病毒中起了关键性的作用。然而,鸡胚法有种种弊端:流感病毒在鸡胚中繁殖和传代,常导致HA分子的抗原结构和病毒受体的特异性改变;加之缺乏高质量的鸡胚,从而严重地限制了鸡 Isolation of Influenza Viruses from Chicken Embryo or Cell Culture is very important in the epidemiological investigation of flu outbreaks and in the development of vaccines. The cell culture of the virus has played a key role in the isolation of the H5N1 bird flu virus that caused the 1997 influenza epidemic in Hong Kong. However, the chicken embryo method has a number of drawbacks: the propagation and passage of the influenza virus in the chicken embryo often leads to the specific alteration of the antigenic structure of the HA molecule and the virus receptor; and the lack of high quality chicken embryo severely limits the chicken